Fig. 4
Live imaging of cell necrosis in cultured wing discs and fat body. a Time lapse imaging of Cry1Aa-induced cell death in wing discs from third instar larvae. Wing discs of WP > GFP, CryR were cultured and Cry1Aa was added to the medium at a final concentration of 200 nM. Images were from just before addition of Cry1Aa or after 20, 40, 60, and 80 min incubation. Full movies are supplied online (Additional file 2: Movie S1, Additional file 3: Movie S2 and Additional file 4: Movie S3). Scale bar, 100 μm. b Time lapse imaging of Cry1Aa-induced cell death in fat body from third instar larvae. Fat bodies of hs-flp 122, UAS-mCD8-GFP; Actin > y > Gal4, UAS-GFP/+; UAS-CryR/+ were incubated with 100 nM Cry1Aa. Flip-out clone cells expressing CryR were labeled with GFP. Images were from 15, 30, and 45 min after incubation. Full movies are supplied online (Additional file 5: Movie S4). Scale bar, 100 μm