Fig. 7From: Induction of rapid and selective cell necrosis in Drosophila using Bacillus thuringiensis Cry toxin and its silkworm receptor In vivo cell ablation of peripheral or central nervous system by Cry1Aa injection. a Adult flies injected with Cry1Aa during third instar larval stage lost their bristles. Arrows in the left panel (negative control, without Neur-Gal4) indicate bristle positions (pDC, posterior Dorsocentral; aSC, anterior Scuteller; pSC, posteior Scuteller) and arrows in the right panel (Neur > CryR) indicate the presence (aSC) or absence (pDC, pSC) of bristles. b Survival curve of male flies with CryR expression throughout the whole body (da) or only in neurons (Elav) with and without Cry1Aa; n = 50 for each condition. c Confocal images of Propidium iodide (PI) staining of larval brain from Elav > CryR, with and without Cry1Aa injection. Brains were dissected 3 h post-injection and then stained with PI. A single focal plane from the middle and surface regions are shown. d Schematic view of selective cell ablation by the Cry1Aa/CryR system. Cry1Aa specifically induces cell swelling and necrosis in CryR-expressing cells by pore formation in the plasma membraneBack to article page