Fig. 3From: Dynamic single cell measurements of kinase activity by synthetic kinase activity relocation sensorsDetermination of MAPK activity from biosensor measurements. a Schematic of the reactions implemented in the model. The SKARS is phosphorylated in the nucleus and in the cytoplasm by MAPK and dephosphorylated by phosphatase. The exchange of SKARS between the cytoplasm and the nucleus can occur through passive diffusion, while only the unphosphorylated SKARS is actively imported in the nucleus. b, c Time-course of activity of MAPK is calculated for different final level of MAPK activity and used as inputs into the model (b). The resulting nuclear-to-cytoplasmic partitioning of the sensor for these different MAPK activity traces are obtained as output (c). d Correlation between MAPK activity and the nuclear-to-cytoplasmic ratio at the end of the simulation. e Dose-response curve of the nuclear enrichment of the sensor as a function of concentration after 15 minutes of stimulation with pheromone (blue circles). The mean and standard deviation of three measurements are plotted. The corresponding MAPK activity level is calculated (red circles). As reference, the nuclear-to-cytoplasmic ratio of the unstimulated non-docking sensor and its corresponding level of MAPK activity are indicated by blue and red squares. f Determination of MAPK activity for the experiment from Fig. 2b. The blue circles correspond to the experimental data points. The dashed blue line represents the fit of those points by the model. The solid red line is the extrapolated MAPK activity for this time-courseBack to article page