Fig. 4
From: Dynamic single cell measurements of kinase activity by synthetic kinase activity relocation sensors
Single cell analysis of MAPK activity upon pheromone stimulation. a Heat map of the response of individual cells to 1 μM α-factor. Each line represents the nuclear-to-cytoplasmic ratio of one single cell normalized by its basal level (Nc = 293). The cell responses were sorted based on the level of final response (measured at T = 12 min). The cells were classified as responding (cyan), non-responding (green), or slow responding (blue). The basal level of each trace is indicated in shades of red (from black to red, increasing basal level). b Scatter plot of initial and final response of the cells. Each dot corresponds to a single cell measurement. The colour indicates to which sub-population the cells belong to. c Temporal evolution of the nuclear-to-cytoplasmic ratio upon pheromone treatment. The solid line represents the mean response of each sub-population. The dashed lines are traces from the three single cells circled in panel (b). d, e, f Images of the selected cells in the brightfield, CFP (histone tag), and RFP (Ste7DS-NLS-RFP) channels at different time points. The dark line in the brightfield image represents the segmented cell contour