Fig. 8
From: Dynamic single cell measurements of kinase activity by synthetic kinase activity relocation sensors
Single cell responses upon zymolyase-induced cell wall damage. a Heat map of the response of individual cells bearing the SKARS Mkk2DS1-100-NLS-RFP to zymolyase (3 U/mL). Each line represents the normalized response of one single cell (Nc = 313). The cell traces were sorted based on the level of final response. This measurement was used to classify the cells in a group of responding cells (blue) and a group of non-responding cells (green). The basal level of each trace is indicated in shades of red (from black to red, increasing basal level). These cells also bear the cell cycle marker Whi5 and its level of nuclear enrichment is indicated in shades of yellow (from black to yellow, increasing nuclear Whi5). b Average nuclear-to-cytoplasmic ratio as function of time for the responding and non-responding cells. c Histogram of the basal level in responding and non-responding cells. d, e Histogram of the final response measured in cells with or without nuclear enrichment of the cell-cycle marker Whi5 (d) or Yox1 (e).