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Fig. 4 | BMC Biology

Fig. 4

From: Functional role of dimerization and CP190 interacting domains of CTCF protein in Drosophila melanogaster

Fig. 4

a Analysis of complexes between dCTCF-CTD and thioredoxin-tagged CP190-BTB mixed at a molar ratio of 1:2 using the chemical cross-linking reagent glutaraldehyde. Proteins were visualized by Coomassie staining. a indicates position of CP190 BTB-domain monomer, b position of dCTCF-CTD, c dimer of CP190 BTB, d complex between CP190 BTB dimer and dCTCF-CTD. b Analysis of stoichiometry of interaction between dCTCF-CTD and CP190-BTB mixed in different molar ratios, and cross-linked with 0.2 % glutaraldehyde after 1 h incubation, visualized by silver-staining. a indicates position of CP190 BTB-domain monomer, b position of CTCF-CTD, c dimer of CP190 BTB, d complex between CTCF-CTD and CP190 BTB in molar ratio 1:2, and e higher order complex between CTCF-CTD and CP190 BTB with unknown stoichiometry. GA glutaraldehyde

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