Fig. 5

a Schematic diagram showing the GE24185 transposon insertion into the dCTCF gene. b Western blots of protein extracts prepared from wild-type and homozygous GE24185 mutant flies. c Schematic representation of dCTCF constructs used to rescue the GE24185 mutation. d Abdomen and cuticle preparations (bottom row) of wild-type and homozygous GE24185 mutant flies in the absence or presence of the hsp83:dCTCF transgenes as indicated. Arrows in GE24185 and dCTCF ^ΔN;GE21485 indicate the presence of a rudimentary A7 tergite and hairs on the A6 sternite. Arrows in dCTCF ^ΔC;GE24185 indicate an A5 to A4 transformation of the tergite. wt wild type, A4-A7 abdominal segments 4-7