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Fig. 4 | BMC Biology

Fig. 4

From: Diversification of non-visual photopigment parapinopsin in spectral sensitivity for diverse pineal functions

Fig. 4

Mutually exclusive localization of PP1 and PP2 in the zebrafish pineal organ. a Expression profiles of PP1 (top panel), PP2 (second panel), UV-sensitive cone opsin (third panel) and β-actin as a reference (bottom panel) in zebrafish. Expected band sizes of PCR fragments of PP1 (270 bp), PP2 (225 bp), UV-sensitive cone opsin (380 bp), and β-actin (386 bp) based on mobility of standard markers are indicated on the left. Immunofluorescent labeling of b PP1 and c PP2 (arrowheads) in the rostral area of the sagittal sections of zebrafish pineal organs. Sections are shown with the dorsal side up, ventral side down, rostral side left and caudal side right. d Schematic drawing of the dorsal view of the zebrafish pineal organ, rostral side left and caudal side right. The red line indicates the approximate region of the sagittal sections in (b) and (c). e Double immunofluorescent labeling of PP1 (magenta) and PP2 (green) shows the mutually exclusive distribution of PP1 and PP2 in the zebrafish pineal organ. The scale bars represent 20 μm

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