Fig. 3

Replication timing of chromosomal features. a Distribution of early and late origins, according to their T₅₀. The percentage of each class is represented on the y axis. Each interval corresponds to 30 s. Early and late origins are defined according to the observed distribution of the two populations of origins, origins firing before 69 min being labeled as early. The distribution of bona fide origins is shown in light orange, and is not statistically different from the whole distribution. b Determination of the average initial replication fork speed. Average fork rates, shown as black dots, are plotted every 400 bp according to their distance from early origins (only bona fide early origins were considered). The orange line corresponds to the linear regression of the first 2 kb. The red line corresponds to the linear regression of the first 5 kb. Correlation coefficients (r²) are indicated near each line. The blue line corresponds to the baseline at which no replication occurs. Intersection of each linear regression with the baseline indicates the amount of DNA replicated within a 5-min time frame (10.5 kb or 15.1 kb) for an average fork speed between 2.1 kb/min and 3 kb/min. c Distribution of replication timing of centromeres and telomeres, according to their T₅₀. Intervals to which mating-type cassettes belong are indicated by blue arrows and histone genes by orange arrows. Note that “Early” and “Late” replication time frames correspond to those defined in a. d Distribution of replication timing of internal chromosomal deletions and chromosomal breakpoints found in translocations, according to their T₅₀. e Distribution of replication timing of subtelomeric and internal megasatellites, according to their T₅₀