Fig. 6

ARS consensus sequences. a C. glabrata ACS determined from the whole set of 275 ARSs (top), or from the 83 bona fide origins (bottom). Boundaries of the element are clearly visible in the latter case. The three positions differing from the S. cerevisiae ACS are shown by orange arrows. The WTW trinucleotide was barely detected. b S. cerevisiae ACS determined from the set of 337 known ARSs listed in the Saccharomyces Genome Database (SGD) [70] (top), or from the 274 ARSs identified here (bottom). Boundaries of the canonical ACS [10] or of the extended ACS (EACS) are indicated, as well as the B1 box and WTW trinucleotide [38]. Note that although the ACS identified in the ARS capture experiment was closer to the C. glabrata ACS than to the S. cerevisiae canonical ACS, the requirement for the B1 box was greater in S. cerevisiae than in C. glabrata. c Alignment of ARS_F6 with the ARS identified by Zordan et al. [46], showing 48 bp in common between the two sequences. The ACS is shown in red. ACS ARS consensus sequence, ARS autonomously replicating sequence