Fig. 4

Stem cell maintenance in zll-1 is restored by reduced ARF2 and increased REV levels. a sqRT-PCR shows that ARF2 transcript levels are strongly downregulated, whereas ARF3 and ARF4 transcripts are unchanged in two independent p35S:amiR-arf2 zll-1 lines compared to untransformed zll-1. b Expression of the shoot meristem stem cell marker pCLV3:GFP-er (green) is discontinued in zll-1 embryos at the bent-cotyledon stage, but recovered in zll-1 expressing p35S:amiR-arf2 and in zll-1 rev-10d double mutants. Scale bars: 20 μm. c Percentage of pCLV3:GFP-er expression levels in given genotypes during embryo development. n number of embryos analyzed. p-values were calculated by the Chi-square test: **p < 0.01, ***p < 0.001