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Fig. 6 | BMC Biology

Fig. 6

From: ZLL/AGO10 maintains shoot meristem stem cells during Arabidopsis embryogenesis by down-regulating ARF2-mediated auxin response

Fig. 6

ARF2 expression is negatively regulated by AGO10 and REV. Quantitative real-time polymerase chain reaction experiments of three independent biological replicates with standard deviation. a ARF2 expression levels in torpedo stage embryos of the indicated genotypes relative to wild-type levels. b pARF2:ntdTomato expression (red) is upregulated in zll-1 heart to bent-cotyledon stage embryos compared to wild type (Ler) and this upregulation is suppressed by the rev-10d mutation. Scale bars: 15 μm for heart and torpedo stage embryos and 30 μm for bent-cotyledon stage embryos. c ARF2 expression levels in 5-day-old shoot apical meristems of the indicated genotypes and treatments. Bars represent the fold changes of ARF2 in response to dexamethasone relative to mock treatment and in response to dexamethasone (DEX) + cycloheximide (CH) relative to CH-control in wild type (Col) and the DEX-inducible 35S::GR-REVd line (GR-REV), respectively. Genotypes and treatments are indicated. Transcription levels are normalized to the reference gene At4g26410. Significance tested by Student’s t-test is indicated. *p < 0.05, **p < 0.01, ***p < 0.001; n.s. not significant

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