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Table 1 Geometry of H-bonds in the GDF-5 R57A:BMPR-IAEC interface

From: GDF-5 can act as a context-dependent BMP-2 antagonist

GDF-5 R57A BMPR-IAEC Distance Å Angle N–O–Ca H-bondb
R18 (NE) G42 (O) 3.19 (−) 162 (−) SC-MC
W33 (NE1) D89 (O) 2.94 (−) 133 (−) SC-MC
L56 (N) Q86 (OE1) 2.88 (2.81) 140 (143) MC-SC
L56 (O) Q86 (NE2) 3.12 (3.19) 137 (140) MC-SC
S58 (N) C77 (O) 2.74 (2.86) 129 (130) MC-MC
S58 (OG) T55 (OG1) 2.93 (2.54) 99 (121) SC-SC
E61 (OE2) K79 (NZ) 3.26 (−) 125 (−) SC-SC
M31 (O) S90 (OG) 2.55 (−) 136 (−) MC-SC
S74 (O) Q94 (N) 2.83 (3.03) 149 (142) MC-MC
S74 (OG) R97 (NH2) - (2.87) - (132) SC-SC
K107 (NZ) D84 (OD2) 3.01 (−) 117 (−) SC-SC
Y109 (OH) D84 (OD2) 2.61 (2.62) 132 (139) SC-SC
Mean value   2.94 (2.84) 131/139 (135/136)  
S.D.   0.21 (0.22) 10 (13)  
  1. A total of 18 intermolecular H-bonds were found in the two interfaces between the GDF-5 dimer and the two BMPR-IA receptors. These non-covalent interactions were formed between 12 atom pairs resulting in an uneven distribution of the 18 H-bonds. The numbers in parentheses are the distances between donor and acceptor atoms and N–O–C angles in the second interface, related by non-crystallographic symmetry
  2. aN, O, C are the donor-acceptor atoms; from general statistics20 this angle is 149 ° ±15 ° for MC–MC hydrogen bonds and 129 ° ±18 ° for SC–MC and SC–SC H-bonds
  3. bMC (main chain) and SC (side chain) donor/acceptor atoms