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Fig. 3 | BMC Biology

Fig. 3

From: Phosphoproteome dynamics mediate revival of bacterial spores

Fig. 3

Characterization of SspA-S47 phosphorylation-site mutants. a Spores of PY79 (wild type, WT), AR209 (sspA-S47A), AR210 (sspA-S47D), and AR179 (∆sspA) strains were incubated at 37 °C in S7-defined medium supplemented with L-Ala (10 mM), and optical density (OD600) was measured at the indicated time points. Data are presented as a fraction of the initial OD600 of the phase-bright spores. Decreasing OD600 signifies spore germination while increasing OD600 indicates spore outgrowth. The data points are averages of results obtained from four independent biological repeats. Error bars designate SD. b Spores of PY79 (wild type, WT), AR209 (sspA-S47A), AR210 (sspA-S47D), and AR179 (∆sspA) strains were exposed to increasing UV (254 nm) doses (mj/cm2) and plated on LB for viable count. Survival was calculated by dividing the viable spore titer at any given UV dose (mj/cm2) with the spore titer obtained from the non-irradiated spores. The data points are averages of results obtained from three independent biological repeats. Error bars designate SD. c Spores of PY79 (wild type, WT), AR209 (sspA-S47A), AR210 (sspA-S47D), and AR179 (∆sspA) strains were germinated with L-Ala (10 mM). Samples were taken at the indicated time points, irradiated with 500 mj/cm2 UV (254 nm), and plated on LB. Survival was calculated by dividing the viable spore titer at any given time point with the spore titer obtained from spores irradiated at the 0 time point. The data points are averages of results obtained from three independent biological repeats. Error bars designate SD. d UV resistance of AR186 (∆sspB), AR195 (∆sspAsspB), AR187 (sspA-S47A sspB), and AR188 (sspA-S47D sspB) spores was determined as described in (b). e UV resistance of AR186 (∆sspB), AR187 (sspA-S47A, ∆sspB), and AR188 (sspA-S47D, ∆sspB) germinating spores was determined as described in (c)

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