Table 5 Glycerophospholipid, non-polar lipid (TG, triacylglycerol; SE, steryl esters), unesterified ergosterol, and free and total fatty acid content in total cell extracts of Pichia pastoris grown on glucose (GAP) or methanol (AOX) as the sole carbon source. Data are listed as μg lipid/mg Wet Cell Weight which have been calculated from at least two independent experiments with standard deviation (±). Significance was estimated by Student’s t-test (two tailed, unpaired)
From: Systems-level organization of yeast methylotrophic lifestyle
| Â | Glucose | Methanol/glycerol | P value |
|---|---|---|---|
Glycerophospholipids | 8.07 ± 0.14 | 8.92 ± 0.30 | 0.01 |
Non-polar lipids | |||
TG | 3.16 ± 0.71 | 1.47 ± 0.28 | 0.06 |
SE | 0.22 ± 0.03 | 0.30 ± 0.03 | 0.07 |
Free ergosterol | 1.67 ± 0.06 | 1.83 ± 0.18 | 0.20 |
Free fatty acids | 2.11 ± 0.49 | 3.38 ± 0.72 | 0.09 |
Total fatty acids | |||
C16:0 | 1.22 ± 0.03 | 1.43 ± 0.16 | 0.01 |
C16:1 | 0.75 ± 0.01 | 0.85 ± 0.06 | 0.003 |
C18:0 | 0.33 ± 0.02 | 0.34 ± 0.09 | 0.67 |
C18:1 | 4.28 ± 0.18 | 3.30 ± 0.31 | 0.0002 |
C18:2 | 3.26 ± 0.14 | 3.45 ± 0.30 | 0.21 |
C18:3 | 0.96 ± 0.04 | 1.30 ± 0.11 | 0.0001 |
∑ of fatty acids | 10.79 ± 0.40 | 10.67 ± 1.01 | 0.79 |