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Fig. 1 | BMC Biology

Fig. 1

From: Mechanisms of blood homeostasis: lineage tracking and a neutral model of cell populations in rhesus macaques

Fig. 1

Probing hematopoietic stem and progenitor cell (HSPC) biology through polyclonal analysis. a Mobilized CD34+ bone marrow cells from rhesus macaques are first marked individually with lentiviral vectors and transplanted back into the animal after nonlethal myeloablative irradiation [19]. Depending on the animal, 30–160 million CD34+ cells were transplanted, with a fraction 0.07–0.3 of them being lentivirus-marked. The clonal contribution of vector-marked HSPCs is measured from blood samples periodically drawn over a dozen years [19]. An average fraction f 0.03–0.1 of the sampled granulocytes and lymphocytes in the peripheral blood was found to be marked. This fraction is smaller than the fraction of marked CD34+ cells due probably to repopulation by surviving unmarked stem cells in the marrow after myeloablative conditioning. Within any post-transplant sample, S=1342–44,415 (average 10,026) viral integration sites of the marked cells were sequenced (see [14, 19] for details). b The fraction of all sequenced VIS reads belonging to each clone is shown by the thickness of the slivers. Small clones are not explicitly shown

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