Fig. 2

Both PPO1 and PPO2 but not PPO3 contribute to injury-related melanization in adults and larvae. Melanization of adults (a) and larvae (b) after clean injury is abolished only in the simultaneous absence of PPO1 and PPO2. A normal melanization spot was observed in the two PPO3 mutants. Arrows indicate the pricking site. Adults and larvae were wounded with a tungsten needle and blackening of the wound was recorded 1 h later in larvae and 16 h later in adults. A representative picture is shown for each genotype