Fig. 4

PPO3 is not required for Toll and Imd pathway activities. a Expression of Diptericin (Dpt) in PPO mutant flies. Total RNA was extracted from animals either uninfected or collected 6 h and 24 h after septic injury with Gram-negative bacteria Erwinia carotovora. Shown are the relative expression levels of Dpt in relation to Rp49. PPO3 ¹ flies show normal induction of Dpt expression levels. The Imd pathway mutant Relish was used as an immune-deficient control. b Expression of Drosomycin (Drs) in PPO3 ¹ mutant flies 24 h and 48 h after septic injury with the Gram-positive bacteria Micrococcus luteus shows that PPO3 ¹ mutant flies have a normal induction of Drs. The Toll pathway mutant spätzle ^rm7 was used as an immune-deficient control. Shown are the relative expression levels of Drs in relation to Rp49. On the graphs, 100 % corresponds to the Dpt (Drs) expression level of wild-types flies 6 h (24 h) after septic injury. Data were analyzed using the Mann–Whitney test. Values represent the mean ± standard deviation of at least three independent experiments. n.s. not significant, UC unchallenged