Fig. 8

Hayan and SP7 do not contribute to melanization of a wasp capsule. Melanization of adults (a) after clean injury is abolished only in the Hayan ^SK3 mutant. A slightly reduced melanization spot was observed in the Sp7 ^SK6 mutant. In contrast, after clean injury of larvae (b), melanization is abolished only in Sp7 ^SK6 whereas Hayan ^SK3 shows residual melanization. Arrows indicate the pricking site. Adults and larvae were wounded with a tungsten needle and blackening of the wound was recorded 1 h later in larvae and 16 h later in adults. After heat-treating larvae (c), black dots, corresponding to crystal cells, appeared in Hayan ^SK3 and Sp7 ^SK6 comparable to wild-type controls. Infested larvae containing eggs of Leptopilina boulardi parasitoid wasps (d) show the presence of a melanized egg in Hayan ^SK3 and Sp7 ^SK6. Arrows indicate the position of the melanized wasp egg. A representative picture is shown for each genotype