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Fig. 8 | BMC Biology

Fig. 8

From: Drosophila innate immunity: regional and functional specialization of prophenoloxidases

Fig. 8

Hayan and SP7 do not contribute to melanization of a wasp capsule. Melanization of adults (a) after clean injury is abolished only in the Hayan SK3 mutant. A slightly reduced melanization spot was observed in the Sp7 SK6 mutant. In contrast, after clean injury of larvae (b), melanization is abolished only in Sp7 SK6 whereas Hayan SK3 shows residual melanization. Arrows indicate the pricking site. Adults and larvae were wounded with a tungsten needle and blackening of the wound was recorded 1 h later in larvae and 16 h later in adults. After heat-treating larvae (c), black dots, corresponding to crystal cells, appeared in Hayan SK3 and Sp7 SK6 comparable to wild-type controls. Infested larvae containing eggs of Leptopilina boulardi parasitoid wasps (d) show the presence of a melanized egg in Hayan SK3 and Sp7 SK6. Arrows indicate the position of the melanized wasp egg. A representative picture is shown for each genotype

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