TY - JOUR AU - Berends, Evelien T. M. AU - Gorham, Ronald D. AU - Ruyken, Maartje AU - Soppe, Jasper A. AU - Orhan, Hatice AU - Aerts, Piet C. AU - de Haas, Carla J. C. AU - Gros, Piet AU - Rooijakkers, Suzan H. M. PY - 2015 DA - 2015/11/09 TI - Molecular insights into the surface-specific arrangement of complement C5 convertase enzymes JO - BMC Biology SP - 93 VL - 13 IS - 1 AB - Complement is a large protein network in plasma that is crucial for human immune defenses and a major cause of aberrant inflammatory reactions. The C5 convertase is a multi-molecular protease complex that catalyses the cleavage of native C5 into its biologically important products. So far, it has been difficult to study the exact molecular arrangement of C5 convertases, because their non-catalytic subunits (C3b) are covalently linked to biological surfaces through a reactive thioester. Through development of a highly purified model system for C5 convertases, we here aim to provide insights into the surface-specific nature of these important protease complexes. SN - 1741-7007 UR - https://doi.org/10.1186/s12915-015-0203-8 DO - 10.1186/s12915-015-0203-8 ID - Berends2015 ER -