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Fig. 1 | BMC Biology

Fig. 1

From: Quantitative framework for ordered degradation of APC/C substrates

Fig. 1

The ABBA motif of Clb5 increases the catalytic rate of ubiquitin transfer. APC/CCdc20 activity was measured with a radiolabeled Clb5-Apc10 fusion protein [14] containing the N-terminal 150 residues of Clb5, either wild-type or carrying the 2A mutations in the ABBA motif. Reactions were performed in the presence of increasing concentrations of E2, and reaction products were subjected to SDS-PAGE and autoradiography with a PhosphorImager. The total amount of ubiquitinated substrate was quantified with ImageQuant software and plotted to determine maximal activity (a.u., arbitrary units). Results are representative of three independent experiments. APC/C anaphase-promoting complex/cyclosome

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