Fig. 3
From: Quantitative framework for ordered degradation of APC/C substrates
A delay in degradation onset and a fast rate of degradation are opposing constraints. a Measurement of T95 as an estimate of the delay in degradation onset, and Td as an estimate of degradation rate. b The k c -k d parameter regions where T95 > 200 sec or Td < 600 sec, as indicated by grey dots. k a  = 0.01/(nM sec). c Center: the parameter region where T95 > 200 sec and Td < 600 sec, as indicated by grey dots. Surrounding plots: substrate degradation profiles with different parameter combinations as indicated by the dotted lines. Black curves show the total amount of substrate; red, pink, and purple curves show the intermediate products with one, two or three ubiquitins attached, respectively. k a  = 0.01/(nM sec). d Incorporation of a deubiquitination reaction (with rate constant k dub ) for all ubiquitinated substrates slightly expands the parameter region with a good delay (T95 > 200 sec) but reduces the region with a fast rate (Td < 600 sec)