Fig. 6

Functions of PIN1 and PIN6 in vein network formation. a-c. Dark-field illumination of mature first leaves illustrating phenotype classes: unbranched, narrow midvein and scalloped vein-network outline (a); bifurcated midvein and scalloped vein-network outline (b); fused leaves with scalloped vein-network outline (c). d. Percentages of leaves in phenotype classes. Difference between RPS5A::PIN1 and WT, between RPS5A::PIN6 and WT, between pin1 and WT, and between RPS5A::PIN1;pin1 and pin1 was significant at P < 0.05 (*), P < 0.01 (**), or P < 0.001 (***) by Kruskal-Wallis and Mann–Whitney test with Bonferroni correction. Sample population sizes: WT, 65; RPS5A::PIN1, 55; RPS5A::PIN6, 58;pin1, 116; RPS5A::PIN1;pin1, 71; RPS5A::PIN6;pin1, 140. e. First leaves. Indices are expressed as mean ± SE. Difference between RPS5A::PIN1 and WT cardinality indices, between RPS5A::PIN6 and WT cardinality indices, between pin1 and WT cardinality indices, between RPS5A::PIN1;pin1 and pin1 cardinality indices, between RPS5A::PIN1 and WT connectivity indices, between pin1 and WT connectivity indices, between RPS5A::PIN1;pin1 and pin1 connectivity indices, and between RPS5A::PIN6;pin1 and pin1 connectivity indices was significant at P < 0.05 (*), P < 0.01 (**), or P < 0.001 (***) by F-test and t-test with Bonferroni correction. Sample population sizes as in (d). Bars: (a-c) 1 mm