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Fig. 4 | BMC Biology

Fig. 4

From: Lack of sex chromosome specific meiotic silencing in platypus reveals origin of MSCI in therian mammals

Fig. 4

RPA dynamics during platypus prophase I. Platypus testis surface spreads were immunostained with anti-RPA32 followed by serial SYCP1 and SMC3 staining as all three antibodies were raised in rabbit. a Abundant RPA32 foci were observed in many nuclei with obvious reductions in nuclei with a large DAPI void typical of cells in advanced stages of prophase I. b RPA32 foci decorate all chromosomes prior to synapsis and SC assembly. As prophase I progresses evidenced by SYCP1 element extension and thickening, RPA32 foci numbers are reduced c and generally absent by the time SMC3 has heavy accumulation on the sex chromosomes d. Scale bar = 5 μm. DAPI 4’6-diamidino-2-phenylindole

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