Skip to main content
Fig. 1 | BMC Biology

Fig. 1

From: iCLIP identifies novel roles for SAFB1 in regulating RNA processing and neuronal function

Fig. 1

Analyses of SAFB1 RNA-interactions by iCLIP. a Western blots showing that following siRNA-mediated knockdown of SAFB1 and SAFB2, the SAFB1 Ab detects a reduction in endogenous SAFB1 but not endogenous SAFB2 and neither does the Ab recognize SAFB2 when mediated by an adenovirus expressing EGFP-tagged SAFB2. Conversely, the SAFB2 antibody (second blot) shows a reduction in endogenous SAFB2 expression following treatment with the SAFB2 siRNA and also detects the EGFP tagged SAFB2 protein. b The bar graphs show the proportion of cDNAs from the iCLIP experiments and their mapping to different genomic regions (human genome hg19) (c) and normalized iCLIP read densities (iCLIP-tags divided by average length of region) mapped to different genomic regions. d SAFB1 crosslink sites mapped around the 3’ and 5’ splice sites from −300 to +300 nucleotides with the splice junction at position 0. Data are normalized by all junctions spanning position, smoothed and represented as 109 × [number of crosslinks]/[total number of crosslinks]. e Z-scores of pentamer occurrence within the 61-nt sequence surrounding crosslink sites calculated by plotting data from individual iCLIP experiments (detailed in the results section). The most enriched pentamers are shown and SAFB1 recognized the GAAGA pentamer with the highest frequency

Back to article page