Skip to main content
Fig. 4 | BMC Biology

Fig. 4

From: iCLIP identifies novel roles for SAFB1 in regulating RNA processing and neuronal function

Fig. 4

SAFB1 regulates splicing from wild type and mutant NCAM1 minigenes. a The distribution of SAFB1 iCLIP tags within the NCAM1 minigene is shown and an arrow indicates the position of exon 9 in transcript variant 5. b The schematic indicates the composition of the minigenes and splice forms generated. The sequence of wild type exon 9, the location of the GAA/AAG and AGA motifs, and the mutations introduced to alter all AAG/AGA/GAA are shown. c PCR analyses using exon-specific spanning primers was used to analyse the effect of SAFB1 knockdown on the expression of the NCAM1 isoforms (NCAM1 7–10, 8–10 and 9–10) in the wild type and mutant minigenes. The open bars represent expression of the transcript indicated with non-targeting control knockdown and the closed bars following SAFB1 knockdown. Values are means ± SEM, statistical analysis was performed by ANOVA and post-hoc t-tests *P <0.05, **P <0.01, ***P <0.001

Back to article page