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Fig. 5 | BMC Biology

Fig. 5

From: iCLIP identifies novel roles for SAFB1 in regulating RNA processing and neuronal function

Fig. 5

Exon array analyses following SAFB1 knockdown. Samples were prepared from SYSY5Y cells following siRNA-mediated SAFB1 knockdown and 1.0 ST Affymetrix high-resolution exon arrays used to analyse effects on splicing. AltAnalyse was used to interrogate the microarray data for alternative splicing events and the significant changes are shown (a). RT-PCR was used to confirm the splicing events identified by arrays (b and c). Primer pairs were generated to distinguish isoforms and three independent experiments were carried out in triplicate. SAFB1 knockdown mediated statistically significant changes in expression of the gene transcripts shown in (b and c). Statistical analysis was by ANOVA followed by post-hoc t-tests. **P <0.01, *P <0.05. B lane 1 control; lane 2 SAFB1 knockdown. Open bars in (c) represent controls, filled bars represent SAFB1 knockdown

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