Fig. 1

Dietary nitrate counteracts the hypoxic suppression of PGC-1α in skeletal muscle and enhances fatty acid oxidation in both normoxic and hypoxic skeletal muscle. (a) Soleus total nitrogen oxide levels (NOx); (b) Fatty acid profile of soleus tissue divided into ≥ C16 (longer-chain) and < C16 (shorter-chain); (c) Carnitine and acyl-carnitine profile of soleus; (d) Respiration rates and respiratory control ratios with palmitoyl-carnitine and malate substrates in permeabilized soleus muscle fibres; (e) 3-hydroxyacyl-CoA dehydrogenase activity in soleus tissue; (f) Malonyl-CoA levels in soleus tissue extracts; (g) Citrate synthase and PGC-1α protein levels in soleus tissue; (h) AMP, ADP and ATP levels in soleus tissue extracts. Data are represented as mean ± SEM, n = 4–10 per experimental group. * P ≤0.05; ** P ≤0.01; *** P ≤0.001