Fig. 4
Nitrate enhances fatty acid oxidation capacity via NO-cGMP-PKG in C2C12 myotubes. (a) Muscle differentiation marker expression in C2C12 myoblasts cultured and differentiated over 6 days; (b) Muscle differentiation marker expression in C2C12 myocytes cultured and differentiated over 6 days in the presence of 0, 50 and 500 μM nitrate. See also Additional file 1: Figure S1. LEAK state respiration of palmitoyl-carnitine and malate substrates in permeabilized C2C12 myotubes cultured and differentiated over 6 days in the presence of (c) 0 or 50 μM nitrate or (d) 0 or 500 μM nitrate, in the presence or absence of sGCi (ODQ, 1 μM) or PKGi (KT5823, 1 μM). OXPHOS state respiration of palmitoyl-carnitine and malate substrates in permeabilized C2C12 myotubes cultured and differentiated over 6 days in the presence of (e) 0 or 50 μM nitrate or (f) 0 or 500 μM nitrate, in the presence or absence of sGCi (ODQ, 1 μM) or PKGi (KT5823, 1 μM). Data are represented as mean ± SEM, n = 4 repeats per condition. * P ≤0.05; ** P ≤0.01; *** P ≤0.001