Skip to main content


Fig. 6 | BMC Biology

Fig. 6

From: Nitrate enhances skeletal muscle fatty acid oxidation via a nitric oxide-cGMP-PPAR-mediated mechanism

Fig. 6

Nitrate and sildenafil cumulatively increase the expression of fatty acid oxidation enzymes and other mitochondrial proteins, and increase CPT1B protein levels. (a) Ucp3, Acad1 and Cpt1b expression in C2C12 myotubes differentiated in the presence of 500 μM nitrate, 1 μM sildenafil, or both nitrate and sildenafil in combination. (b) CPT1B protein levels in C2C12 cells exposed to 500 μM nitrate, 1 μM sildenafil, nitrate and sildenafil in combination, 1 μM ODQ, and nitrate and ODQ in combination. Data are represented as mean ± SEM, n = 3 repeats per condition. * P ≤0.05; ** P ≤0.01; *** P ≤0.001

Back to article page