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Fig. 4 | BMC Biology

Fig. 4

From: The structure of a prophenoloxidase (PPO) from Anopheles gambiae provides new insights into the mechanism of PPO activation

Fig. 4

E364 is key to PO enzymatic catalysis. (a) E364Q mutation severely impairs diphenol oxidase activity of AgPPO8. Dopamine was used as the substrate and specific PO activity was shown as ΔmOD470/min/μg or U/μg; P <0.001. (b) E364Q mutation severely impairs the monophenol hydroxylase activity of AgPPO8. Tyramine was used as the substrate and the rate of dopamine formation catalyzed by 1 μg enzyme was estimated as ΔmOD280/min/μg or U/μg; P <0.001. All data were presented as mean ± SEM (n = 3)

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