Fig. 4

The microtubule network is stabilized by tankyrase 1 and 2 (TNKS/2) pharmacological neutralization. a Confocal images and b morphometric quantitation of the microtubule network at standard temperature (37 °C) or after cold (4 °C) exposure. Starved A549 cells were pre-incubated for 24 h with or without TNKS/2 inhibitors (10 μM) and then placed on ice for 6 min (cold treatment) before immediate fixation and staining with anti-α-tubulin antibody. Scale bar, 7 μm. Images are representative of one experiment out of two performed. A minimum of 52 cells from at least two different fields were analyzed for content of polymerized tubulin. ***P < 0.001 by two-tailed Student’s t-test. NS not significant. Raw data for panel b are shown in Additional file 13. DMSO dimethyl sulfoxide