Skip to main content
Fig. 6 | BMC Biology

Fig. 6

From: Tankyrase inhibition impairs directional migration and invasion of lung cancer cells by affecting microtubule dynamics and polarity signals

Fig. 6

Tankyrase 1 and 2 (TNKS/2) inhibitors delay the formation of cortical adenomatous polyposis coli (APC) clusters in migrating cells. a Confocal images of serum-starved, wound-edge A549 cells, either untreated or treated with TNKS/2 inhibitors (10 μM) and then subject to a time course of hepatocyte growth factor (HGF) stimulation (HGF, 50 mg/mL). Cells were fixed and stained with anti-APC antibody. b Quantitation of APC-decorated protrusions. The curves represent APC enrichment in cortical areas over time. Results are expressed as the percentage of APC-positive protrusion ± standard error of the mean. A minimum of 163 cells was counted for each experimental condition in three independent experiments (P < 0.001 by two-way analysis of variance). Scale bar, 7 μm. Raw data for panel b are shown in Additional file 17. DMSO dimethyl sulfoxide

Back to article page