Fig. 6
From: Serotonin promotes exploitation in complex environments by accelerating decision-making
Optogenetic activation of serotonergic neurons induces a rapid slowdown off food. The temporal dynamics of motion were assayed using the frame difference method (i.e. the number of pixels that changed their value between consecutive frames of a movie of a single animal; see Methods). Three independent lines (two lite-1 backgrounds and one lite-1; mod-5 background) expressing ChR2 in both NSM and ADF serotonergic neurons (Ptph-1::ChR2) were assayed. Control animals (lite-1) lacked ChR2 or TPH-1. The shaded area depicts the period in which the blue light was on. Inset: single exponential fits to each dataset (see Methods). Plots depict mean ± SEM, the number of stimuli assayed is noted in parentheses for each condition, the recovery timescale of lite-1; mod-5 double mutants was compared to that of lite-1 mutants using an ANOVA test corrected post hoc for multiple comparisons using Tukey’s HSD test, and double asterisks denote significant differences (P <0.01)