Skip to main content
Fig. 2 | BMC Biology

Fig. 2

From: Detection of pup odors by non-canonical adult vomeronasal neurons expressing an odorant receptor gene is influenced by sex and parenting status

Fig. 2

The Olfr692 OR gene is expressed in a large subpopulation of cells in the adult mouse vomeronasal organ. (a) In situ hybridization (ISH) on 16-μm cryostat VNO sections with a cRNA DIG-labeled probe for Olfr692. Chromogenic development stains a defined subpopulation of sparsely distributed cells (purple), along the anterior-posterior VNO axis, with no apparent spatial segregation (representative images from a set 48 sections, from 20 mice). (b) Chromogenic ISH on a VNO section with DIG-labeled probe for Olfactory Marker Protein (OMP), coding for OMP, expressed in all VSNs. (c) Fluorescent ISH on VNO sections with DIG-labeled probe for Olfr692 (red fluorescence; representative images from a set of 20 sections, from 20 individuals). (d) Fluorescent ISH with DNP-labeled probe for Vmn2r118 (green). See Additional file 1: Figure S1b–d for other V2R receptors. (e) Quantitation of Olfr692-positive cells in apical (blue bars) or basal (red bars) zones of the VNO (error bars are SEM; n = 41 subjects; two to three sections per subject). (f) Chromogenic ISH on an MOE section with DIG-labeled Olfr692 probe (representative images from a set of nine sections, from three individuals). ep, sensory epithelium; lu, vomeronasal organ lumen; ns, non-sensory tissue and blood vessels. Scale bars represent 100 μm. Blue labeling indicates To-Pro-3 nuclear staining. See also results with control OR probes in Additional file 1: Figure S1a

Back to article page