Enzyme | Fasted state | Fed state | Enzyme | Fasted state | Fed state |
|---|
4 mM glucose a
| 10 mM glucose b
| 4 mM glucose b
| 10 mM glucose a
| 4 mM glucose a
| 10 mM glucose b
| 4 mM glucose b
| 10 mM glucose a
|
|---|
ALD |
0.00
| 0.00 | 0.00 |
0.00
| MDHmito
|
0.00
| 0.00 | 0.00 |
0.00
|
EN |
0.00
| 0.00 | 0.00 |
0.00
| NDKUTP
|
0.00
| 0.00 | 0.00 |
0.00
|
FBP1 |
0.01
| –0.03 | 0.04 |
0.00
| NDKGTP
|
0.00
| 0.00 | 0.00 |
0.00
|
FBP2 |
0.00
| –2.01 | 0.12 |
–0.18
| NDKmito(GTP)
|
0.00
| 0.00 | 0.00 |
0.00
|
GAPDH |
0.00
| 0.00 | 0.00 |
0.00
| PC |
0.66
| –0.25 | 0.60 |
–0.01
|
GK |
0.00
| 2.87 | –0.05 |
0.56
| PEPCK |
0.00
| 0.00 | 0.00 |
0.00
|
GlcT |
0.00
| 0.27 | 0.04 |
0.54
| PEPCKmito
|
0.00
| 0.00 | 0.00 |
0.00
|
GlcTER
|
0.00
| 0.00 | 0.00 |
0.00
| PEPT |
0.00
| 0.00 | 0.00 |
0.00
|
GP |
–0.04
| 0.11 | –0.08 |
0.00
| PFK1 |
0.00
| 0.22 | 0.00 |
0.01
|
G6P |
0.06
| –2.34 | 0.30 |
–0.12
| PFK2 |
0.00
| 2.01 | –0.12 |
0.18
|
GPI |
0.00
| 0.00 | 0.00 |
0.00
| PGK |
0.00
| 0.00 | 0.00 |
0.00
|
G6PTER
|
0.00
| 0.00 | 0.00 |
0.00
| PGM |
0.00
| 0.00 | 0.00 |
0.00
|
GS |
0.04
| –0.15 | 0.06 |
0.00
| PK |
–0.01
| 0.28 | –0.06 |
0.01
|
LacT |
0.28
| 0.01 | 0.14 |
0.00
| PyrT |
0.00
| 0.00 | 0.00 |
0.00
|
LDH |
0.00
| 0.00 | 0.00 |
0.00
| PyrMalT |
0.00
| 0.00 | 0.00 |
0.00
|
MDH |
0.00
| 0.00 | 0.00 |
0.00
| TPI |
0.00
| 0.00 | 0.00 |
0.00
|
- For the numerical calculation of the control coefficients the amounts of the catalyzing enzymes were varied by 5 % (0.5 % yielded similar results). The sum of all control coefficients is equal to unity (summation theorem) within the limit of numerical accuracy
-
a In bold font are the control coefficients for the physiological states (i.e. fasted protein profile and 4 mM plasma glucose and low glycogen store and fed protein profile and 10 mM blood glucose and high glycogen store)
-
b In normal font are the control coefficients for the two states with the respective other protein profile. The reference flux values are –64 μmol/g/h for fasted hepatocytes and 81 μmol/g/h for fed hepatocytes
-
ALD Aldolase, EN Enolase, ER Endoplasmic reticulum, FBP1 Fructose-1,6-bisphosphatase, FBP2 Fructose-2,6-bisphosphatase, GAPDH Glyceraldehyde 3-phosphate dehydrogenase, GK Glucokinase, GlcT Glucose transporter, G6P Glucose-6-phosphate phosphatase, GPI Glucose-6-phosphate isomerase, G6PT Glucose-6-phosphate transporter, GTP Guanosine triphosphate, GS Glycogen synthetase, LacT Lactate transporter, LDH Lactate dehydrogenase, MDH Malate dehydrogenase, mito Mitochondrion, NDK Nucleoside-diphosphate kinase, PyrMalT Pyruvate/malate antiporter, PC Pyruvate carboxylase, PEPCK Phosphoenolpyruvate carboxykinase, PEPT Phosphoenolpyruvate transporter, PFK1/2 Phosphofructokinase-1/2, PGK Phosphoglycerate kinase, PGM Phosphoglycerate mutase, PK Pyruvate kinase, PyrT Pyruvate transporter, TPI Triose-phosphate isomerase, UTP Uridine triphosphate
