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Fig. 1 | BMC Biology

Fig. 1

From: An La-related protein controls cell cycle arrest by nuclear retrograde transport of tRNAs during diapause formation in Artemia

Fig. 1

Progression and characterization of cell cycle arrest during Artemia diapause embryo formation and termination. a Developmental stages of Artemia during diapause formation (oviparous pathway) and direct development (ovoviviparous pathway). 1, Pre-diapause (early embryos); 2, diapause; 3, post-diapause; 4, nauplii; 1', early embryos; 4', nauplii. Scale bar = 1 mm. b Expression of the mitosis markers CDK6, cyclin D3, phosphorylated Rb (Thr356), and phosphorylated histone H3 (Ser10) at various stages of development. The lane numbers correspond to the developmental stages shown in (a). Histone H3 (H3) and α-tubulin were used as the loading controls for the nucleus and cytoplasm, respectively. c Analysis of the cell cycle phase during various stages of development. Flow cytometry analysis was performed with a fixed cell suspension stained with PE at each stage. The right panel shows the DNA content of cells in each stage during diapause formation. d 5-bromo-2’-deoxyuridine (BrdU) incorporation assay and e immunofluorescence of the proliferation markers Ki67 and proliferating cell nuclear antigen at each stage of Artemia during diapause formation. Scale bar = 500 μm

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