Fig. 2

Ar-Larp gene expression in each developmental stage of Artemia in the oviparous and ovoviviparous pathways. a Real-time quantitative PCR analysis of Ar-Larp expression during each developmental stage (indicated in Fig. 1a). Error bars represent the mean ± SD of three independent experiments. b Western blot analysis of Ar-Larp during each developmental stage using α-tubulin as the loading control. c Subcellular location of Ar-Larp. Western blot analysis of Ar-Larp in the cytoplasm and nucleus during each developmental stage of the oviparous pathway. Histone H3 (H3) and α-tubulin served as the loading controls for the nuclear and cytoplasmic samples, respectively