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Fig. 3 | BMC Biology

Fig. 3

From: An La-related protein controls cell cycle arrest by nuclear retrograde transport of tRNAs during diapause formation in Artemia

Fig. 3

RNA interference (RNAi) of Ar-Larp in Artemia. a The expression levels of Ar-Larp mRNA were determined by real-time quantitative PCR after RNAi treatments. Int, intact; Ctrl, control treatment with 800 ng of GFP double stranded RNA (dsRNA). The proteins in diapause cysts were analyzed by Western blotting and tubulin was used as the loading control. b The phenotypes of RNAi-treated cysts injected with 800 ng of Ar-Larp dsRNA. Observation by the anatomical microscopy. Scale bar = 500 μm. Embryos treated with GFP dsRNA remained in diapause, whereas those treated with Ar-Larp dsRNA entered a pseudodiapause and developed into nauplii. c Western blot analysis of CDK6, cyclin D3, p26, Artemin, phosphorylated Rb (Thr356 and Ser807/811), and phosphorylated histone H3 (Ser10) after RNAi of Ar-Larp. Histone H3 (H3) and α-tubulin were used as the loading controls for the nucleus and cytoplasm, respectively

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BMC Biology

ISSN: 1741-7007

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