Fig. 6

Ar-Larp inhibits mitosis of HeLa and HT1080 cells in mouse xenograft tumor models. a Female BALB/c nude mice were intratumorally treated with Ad-Ar-Larp or Ad-control after the formation of HeLa or HT1080 tumors. Tumor volumes and images are shown. The error bars represent the mean ± SD for five animals. Ar-Larp expression in the tumors was detected by Western blotting (insert). b Mitotic activity of cells in HeLa and HT1080 tumors examined using the BrdU incorporation assay after treatment with Ad-Ar-Larp or Ad-control. Blue, nuclei counterstained with DAPI. Green, Ar-Larp detected with a FITC-conjugated anti-Ar-Larp antibody. Red, incorporated BrdU detected with a rhodamine-conjugated anti-digoxigenin antibody. There is no mitotic activity in the green cells in the merged fields. Scale bar = 50 μm. c Expression of Ar-Larp and mitosis markers including CDK6, cyclin D3, and phosphorylated Rb (Thr356, Ser780, and Ser807/811). Tubulin was used as a loading control