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Fig. 1 | BMC Biology

Fig. 1

From: The role of the aryl hydrocarbon receptor in the development of cells with the molecular and functional characteristics of cancer stem-like cells

Fig. 1

Ahr and Cyp1b1 expression is increased in ALDH1high Hs578T cells. (a) ER/PR/HER2 Hs578T cells were stained with ALDEFLUOR™ in the presence or absence of diethylaminobenzaldehyde (DEAB), a specific ALDH inhibitor, and ALDH activity (production of fluorescent substrate) was quantified by flow cytometry. Regions were set using dot plots from DEAB-treated cells. Data are representative of 24 experiments. (b) Ahr and Cyp1b1 mRNA expression in sorted ALDHhigh and ALDHlow cells was quantified by RT-qPCR. Data from three independent experiments were analyzed using the Pfaffl method [103], normalized to the Gapdh signal, and presented as mean fold-change from ALDHlow ± standard error. Asterisks indicate a significant increase in the mRNA fold-change, *P <0.05, **P <0.005

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