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Fig. 7 | BMC Biology

Fig. 7

From: The role of the aryl hydrocarbon receptor in the development of cells with the molecular and functional characteristics of cancer stem-like cells

Fig. 7

AHR down-regulation decreases Hs578T tumorsphere formation. (a) Dox-inducible shAhr-transduced Hs578T (‘shAhr’) or wildtype Hs578T cells were left untreated or treated for 48 hours with vehicle, doxycycline, or 10 μM CH223191 as indicated and cultured in Mammocult media under ultra-low adherence conditions. Representative images of primary (day 8) and secondary (day 16, following passage at day 8) tumorspheres are presented. The percentage of viable cells is included on each image. Vehicle and CH223191 treatment groups are representative of six independent experiments. shAHR, no dox and shAHR + dox treatment groups are representative of five independent experiments. (b) Hs578T cells were treated as in (a) and tumorsphere formation was quantified. Data from six experiments were normalized to results obtained with naïve cells and presented as mean fold-changes from naive ± standard error. Asterisks indicate a significant decrease in the percentage of tumorspheres, *P <0.05, **P <0.005

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