Fig. 2
From: Mesodermal ALK5 controls lung myofibroblast versus lipofibroblast cell fate
Mesodermal Alk5 deficiency inhibits smooth muscle development. (a–h) Immunohistochemistry (IHC) showed αSMA is decreased throughout lung development in Alk5 Dermo1 lungs. (i) Quantitative PCR (Q-PCR) confirmed the repression of myofibroblast-related mRNAs in Alk5 Dermo1 lungs at E13.5. n = 2 pairs of separate lungs, repeated once. (j) Q-PCR analysis showed decreased Noggin with smaller loss in Heyl expression in Alk5 Dermo1 lungs at E13.5. n = 3 pairs of separate lungs. (k) Q-PCR showed decreased Tnc and Prrx1 mRNA in Alk5 Dermo1 lungs. n = 3 pairs of separate lungs. Error bars show standard deviation. *P <0.05. Scale bar: h = 20 μm