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Fig. 3 | BMC Biology

Fig. 3

From: Mesodermal ALK5 controls lung myofibroblast versus lipofibroblast cell fate

Fig. 3

Mesodermal Alk5 deficiency reduces smooth muscle precursors. (af) Immunohistochemistry showed reduced αSMA (green) and PDGFRα (red) in Alk5 Dermo1 lungs throughout lung development. (g) Western blot analysis showed decreased αSMA, PDGFRα and p-AKT protein in E15.5 Alk5 Dermo1 whole lung tissue. β-ACTIN was used as control. n = 3 separate lungs. (h) Western blot analysis showed decreased p-AKT protein in primary mesenchymal cells isolated from E15.5 Alk5 Dermo1 lungs. n = 3 pairs of separate lungs. β-ACTIN was used as control. (i) Densitometric analysis of western blot results in (g). Error bars show standard deviation. *P <0.05. Scale bar: f = 20 μm

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