Fig. 1
From: The transcription factor scleraxis is a critical regulator of cardiac fibroblast phenotype
Scleraxis up-regulates matrix target genes. a–c Assay of collagen (a), proteoglycan (b) and matrix metalloproteinase (c) mRNA expression by qPCR following over-expression of Scx (AdScx) in primary rat cardiac proto-myofibroblasts compared to controls (AdGFP) reveals numerous matrix genes are induced; n = 3. d Protein lysates from AdGFP- or AdScx-infected primary rat cardiac proto-myofibroblasts were assayed for MMP activity by gel zymography. Zymograms were obtained 24 or 48 h following infection and revealed transient induction of proMMP2 and loss of MMP9 following Scx over-expression; C, control recombinant human MMP2 protein; n = 3. *P < 0.05 vs AdGFP, #P < 0.05 vs corresponding 24 h time point