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Fig. 2 | BMC Biology

Fig. 2

From: The transcription factor scleraxis is a critical regulator of cardiac fibroblast phenotype

Fig. 2

Cardiac matrix gene expression is attenuated by scleraxis knockdown. a Primary cardiac proto-myofibroblasts exhibited loss of Scx but not paraxis mRNA following infection with adenovirus encoding an shRNA targeting Scx (AdshScx) but not control shRNA (AdshLacZ) for 72 h (assayed by qPCR). Results were normalized to the respective AdshLacZ sample; n = 3. b Cells treated as in (a), with or without 10 ng/mL TGFβ or vehicle, were assayed for Scx protein expression 72 h after infection and results normalized to α-tubulin; n = 4. AdshScx attenuated Scx protein expression even in the presence of TGFβ. cd Fibrillar collagen mRNA (c) and nascent soluble 150 kDa collagen I protein (d) expression was down-regulated following Scx knockdown (assayed by qPCR or western blotting, respectively), with cell treatment and normalization as in (a); n = 3. ef The expression of mRNAs encoding several proteoglycans (e) and matrix metalloproteinases (f) (assessed by qPCR) was reduced following Scx knockdown; n = 3. *P < 0.05 vs corresponding AdshLacZ control

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