Fig. 3
From: The transcription factor scleraxis is a critical regulator of cardiac fibroblast phenotype
Scleraxis regulates cardiac fibroblast phenotype. a–c Fibroblast/myofibroblast marker gene mRNA (a, c) and protein (b) expression was assayed in primary cardiac proto-myofibroblasts following Scx over-expression (AdScx) vs controls (AdGFP) (a, b), or following Scx knockdown (AdshScx) compared to controls (AdshLacZ) (c), assayed by qPCR or western blot; n = 3. Scx loss induced similar down-regulation of fibroblast and myofibroblast markers, while Scx over-expression induced marker expression. d Scx transactived the vimentin, MMP2 and fibronectin gene promoters compared to empty vector control (C) as determined by luciferase reporter assays in NIH-3T3 fibroblasts; n = 3 (vimentin, fibronectin) or n = 4 (MMP2). *P < 0.05 vs AdGFP (a, b), vs AdshLacZ (c) or vs control empty vector (d)