Fig. 3From: Dynamics of BMP and Hes1/Hairy1 signaling in the dorsal neural tube underlies the transition from neural crest to definitive roof plateDynamics of BMP responsive element (BRE) in the dorsal neural tube (NT). a–c Hemi-NT electoporations of BRE-GFP along with control RFP to trace electroporation efficiency. Embryos were electroporated and analyzed at designated times. Note presence of positive BRE-GFP signal in RFP+ cells in the early electroporations (a–b”). When electroporated later (c–c”), the BRE-GFP signal is absent in the definitive roof plate (RP) but positive more ventrally. Dashed lines in b and c delimit the definitive RP (see “Methods”). d–d” In situ hybridization for Foxd3 transcripts, showing co-localization with the BRE-GFP signal in the dorsal NT. Dashed lines mark the ventral limit of the Foxd3-positive domain. Note also the presence of Foxd3+ neural crest cells migrating dorso-ventrally outside the neuroepithelium (arrows). In addition, the BRE-GFP signal also extends further ventrally into a Foxd3-negative region. Bar for a–a” = 50 μM; b–d” = 40 μMBack to article page