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Fig. 3 | BMC Biology

Fig. 3

From: Dynamics of BMP and Hes1/Hairy1 signaling in the dorsal neural tube underlies the transition from neural crest to definitive roof plate

Fig. 3

Dynamics of BMP responsive element (BRE) in the dorsal neural tube (NT). ac Hemi-NT electoporations of BRE-GFP along with control RFP to trace electroporation efficiency. Embryos were electroporated and analyzed at designated times. Note presence of positive BRE-GFP signal in RFP+ cells in the early electroporations (ab”). When electroporated later (cc”), the BRE-GFP signal is absent in the definitive roof plate (RP) but positive more ventrally. Dashed lines in b and c delimit the definitive RP (see “Methods”). dd” In situ hybridization for Foxd3 transcripts, showing co-localization with the BRE-GFP signal in the dorsal NT. Dashed lines mark the ventral limit of the Foxd3-positive domain. Note also the presence of Foxd3+ neural crest cells migrating dorso-ventrally outside the neuroepithelium (arrows). In addition, the BRE-GFP signal also extends further ventrally into a Foxd3-negative region. Bar for aa” = 50 μM; bd” = 40 μM

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