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Fig. 6 | BMC Biology

Fig. 6

From: Dynamics of BMP and Hes1/Hairy1 signaling in the dorsal neural tube underlies the transition from neural crest to definitive roof plate

Fig. 6

The relationship between Hes1/Hairy1, BMP signaling, and downstream Foxd3 expression. ab” Embryos were electroporated with either control GFP (aa”) or Hes1 (bb”) at the neural crest (NC) stage and analyzed 20 h later for pSMAD immunostaining (red). Note the decreased pSMAD signal in the treated side at b’ (arrow) compared to the untransfected side and to control GFP. cd’ Embryos were electroporated with either control GFP (c, c’) or Hes1/GFP (d, d’) at the NC stage and analyzed 15 h later for Alk3 mRNA expression. Note the reduction in the Alk3 signal in the transfected (green) side of the Hes-treated hemi-neural tube (NT) (white arrow). ee” Mutually exclusive spatial expression domains of Foxd3 and Hairy1. In situ hybridization on adjacent sections of the same embryo performed at 35 ss. Note that at this particular stage, Hairy1 expression (blue) is ventral to the Foxd3+ (black) domain. f, g’ Foxd3 and Hairy1 inhibit each other’s expression. Electroporation of Foxd3 at E2.5 cell autonomously inhibits Hairy1 expression in the dorsal NT 16 h later (g, g’; arrowheads), compared to the contralateral side and to GFP-electroporated controls (f, f’; arrows). h, h’ Electroporation of Hes1 at the NC stage inhibits Foxd3 transcription compared to the contralateral side. Arrowheads point to GFP+/Foxd3− cells. For control see Fig. 4f. Bar for ab”, fg’ = 60 μM; ce”, h, h’ = 50 μM

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