Fig. 2

Hmga2 suppression interferes with the transition of embryonic stem cells (ESCs) into epiblast-like stem cells (EpiLCs). a qPCR analysis to compare the expression levels of the indicated genes in ESCs versus EpiLCs. Data are reported as means (n = 3) ± SEM of biological replicates. b Comparison of the gene expression changes of ESC (Rex1 and Klf4) and EpiLC (Fgf5 and Oct6) markers in ESCs and EpiLCs upon Hmga2 silencing. Data are reported as means (n = 3) ± SEM of biological replicates (in the case of Fgf5 and Oct6 in EpiLC n = 4). c Western blot analysis to measure the expression level of Oct4, Nanog, and Hmga2 in EpiLCs derived from ESCs transfected with the indicated siRNAs. d Gene expression changes as a consequence of Hmga2 knockdown in EpiLCs transfected with GFP (mock) or Hmga2 under the control of the β-actin gene promoter. qPCR data are reported as mean of relative mRNA expression ± SEM of biological replicates (Fgf5 and Rex1, n = 4; Oct6 and Klf4, n = 3). *P <0.05, **P <0.01, ns: not significant